Cellartis DEF-CS 500 Culture System

产品编号  Y30010 | TAKARA CLONTECH
DEF-CS Culture System for Expanding Human Induced Pluripotent Stem Cells
The DEF-CS system is a robust culture system for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder-free and defined environment. This system enables a stable growth rate that is equally ideal for traditional iPS cell culture, mass production of cells, and single cell culture. Cells grown with this culture system are maintained in an undifferentiated state with virtually no detectable background differentiation, eliminating the need for cell selection. Enzymatic passaging as single cells allows for single-cell applications, including high-throughput screening, transfection, and scaffold seeding.
产品描述
Features
?	Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
?	Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
?	Complete kit includes 500 ml basal medium, additives, and coating compound
?	Robust system provides high reproducibility and stable growth
?	Maintains cells in an undifferentiated state with virtually no background differentiation
Applications
?	Scale-up and mass production of human iPS cells
?	Single-cell culture of human iPS cells
?	Transfection and reprogramming
?	High-throughput screening
?	Tissue engineering (seeding cells on a scaffold)
Published Examples of Use
1.	Borestr?m, C. et al. (2014) Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source. Stem Cells Trans. Med. 3:433–447.
2.	Hanson, C. et al. (2013) Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91(2):127–130.
3.	Kia, R. et al. (2014) MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicological Sciences kfu269.
4.	Norrman, K. et al. (2013) Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59(1):59–70.
5.	Sivertsson, L. et al. (2012) Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor.  Stem cells and development 22(4):581–594.
6.	Ulvestad, M. et al. (2013) Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells.Biochemical pharmacology 86(5):691–702.
7.	Valton, J. et al. (2014) Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69(2):151–170.
8.	Zandén, C. et al. (2014) Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. Nanomedicine: Nanotechnology, Biology and Medicine10(5):949–958.
Additional Information
?	Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美**家，也是亚裔创办的较大的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠**。Uante,您的科研物资战略合作伙伴！
重庆市寰宇安泰生物科技有限公司 
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